T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS
Identifieur interne : 005A56 ( Main/Exploration ); précédent : 005A55; suivant : 005A57T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS
Auteurs : Yue-Dan Wang [République populaire de Chine, Hong Kong] ; Wan-Yee Fion Sin [Hong Kong] ; Guo-Bing Xu [République populaire de Chine] ; Huang-Hua Yang [Danemark] ; Tin-Yau Wong [Danemark] ; Xue-Wen Pang [République populaire de Chine] ; Xiao-Yan He [République populaire de Chine] ; Hua-Gang Zhang [République populaire de Chine] ; JOICE NA LEE NG [Danemark] ; Chak-Sum Samuel Cheng [Hong Kong] ; JING JU [Hong Kong] ; LI MENG [Hong Kong] ; Rui-Feng Yang [République populaire de Chine] ; Sik-To Lai [Danemark] ; Zhi-Hong Guo [Danemark] ; YONG XIE [Hong Kong] ; Wei-Feng Chen [République populaire de Chine]Source :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Antigène HLA-A2 (analyse), Données de séquences moléculaires, Déterminants antigéniques des lymphocytes T, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Interféron gamma (biosynthèse), Lymphocytes T (immunologie), Protéines de l'enveloppe virale (immunologie), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Virus du SRAS (immunologie).
- MESH :
- analyse : Antigène HLA-A2.
- biosynthèse : Interféron gamma.
- immunologie : Glycoprotéines membranaires, Lymphocytes T, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Virus du SRAS.
- Pascal (Inist)
- Coronavirus, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes T, Glycoprotéine de spicule des coronavirus, Homme, Humains, Lymphocyte T, Déterminant antigénique, Protéine A, Immunité cellulaire, Microbiologie, Séquence d'acides aminés, Virologie, Syndrome respiratoire aigu sévère.
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antigenic determinant, Cellular immunity, Coronavirus, Epitopes, T-Lymphocyte, HLA-A2 Antigen (analysis), Human, Humans, Interferon-gamma (biosynthesis), Membrane Glycoproteins (immunology), Microbiology, Molecular Sequence Data, Protein A, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, T-Lymphocyte, T-Lymphocytes (immunology), Viral Envelope Proteins (immunology), Virology.
- MESH :
- chemical , analysis : HLA-A2 Antigen.
- chemical , biosynthesis : Interferon-gamma.
- chemical , immunology : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical : Epitopes, T-Lymphocyte, Spike Glycoprotein, Coronavirus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- Amino Acid Sequence, Humans, Molecular Sequence Data.
Abstract
The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-γ) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-γ-secreting T-cell response in HLA-A2+ donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2+ healthy donors or in HLA-A2- donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HLA-A2+ SARS-CoV-infected patients.
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Le document en format XML
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Wen" uniqKey="Pang X" first="Xue-Wen" last="Pang">Xue-Wen Pang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="He, Xiao Yan" sort="He, Xiao Yan" uniqKey="He X" first="Xiao-Yan" last="He">Xiao-Yan He</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Hua Gang" sort="Zhang, Hua Gang" uniqKey="Zhang H" first="Hua-Gang" last="Zhang">Hua-Gang Zhang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Joice Na Lee Ng" sort="Joice Na Lee Ng" uniqKey="Joice Na Lee Ng" last="Joice Na Lee Ng">JOICE NA LEE NG</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel 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SARS</title><author><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Sin, Wan Yee Fion" sort="Sin, Wan Yee Fion" uniqKey="Sin W" first="Wan-Yee Fion" last="Sin">Wan-Yee Fion Sin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Guo Bing" sort="Xu, Guo Bing" uniqKey="Xu G" first="Guo-Bing" last="Xu">Guo-Bing Xu</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>First Hospital, Peking University</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Yang, Huang Hua" sort="Yang, Huang Hua" uniqKey="Yang H" first="Huang-Hua" last="Yang">Huang-Hua Yang</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>DNK</s3><sZ>4 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Pang, Xue Wen" sort="Pang, Xue Wen" uniqKey="Pang X" first="Xue-Wen" last="Pang">Xue-Wen Pang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="He, Xiao Yan" sort="He, Xiao Yan" uniqKey="He X" first="Xiao-Yan" last="He">Xiao-Yan He</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Hua Gang" sort="Zhang, Hua Gang" uniqKey="Zhang H" first="Hua-Gang" last="Zhang">Hua-Gang Zhang</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Joice Na Lee Ng" sort="Joice Na Lee Ng" uniqKey="Joice Na Lee Ng" last="Joice Na Lee Ng">JOICE NA LEE NG</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Jing Ju" sort="Jing Ju" uniqKey="Jing Ju" last="Jing Ju">JING JU</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Li Meng" sort="Li Meng" uniqKey="Li Meng" last="Li Meng">LI MENG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Rui Feng" sort="Yang, Rui Feng" uniqKey="Yang R" first="Rui-Feng" last="Yang">Rui-Feng Yang</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>First Hospital, Peking University</s1><s2>Beijing</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Princess Margaret Hospital</s1><s3>DNK</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Zhi Hong" sort="Guo, Zhi Hong" uniqKey="Guo Z" first="Zhi-Hong" last="Guo">Zhi-Hong Guo</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>DNK</s3><sZ>4 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay</s1><s2>Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>16 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Immunology, Peking University Health Science Centre</s1><s2>Beijing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antigenic determinant</term><term>Cellular immunity</term><term>Coronavirus</term><term>Epitopes, T-Lymphocyte</term><term>HLA-A2 Antigen (analysis)</term><term>Human</term><term>Humans</term><term>Interferon-gamma (biosynthesis)</term><term>Membrane Glycoproteins (immunology)</term><term>Microbiology</term><term>Molecular Sequence Data</term><term>Protein A</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe acute respiratory syndrome</term><term>Spike Glycoprotein, Coronavirus</term><term>T-Lymphocyte</term><term>T-Lymphocytes (immunology)</term><term>Viral Envelope Proteins (immunology)</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigène HLA-A2 (analyse)</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes T</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Interféron gamma (biosynthèse)</term><term>Lymphocytes T (immunologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>HLA-A2 Antigen</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigène HLA-A2</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron gamma</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Lymphocytes T</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes T</term><term>Glycoprotéine de spicule des coronavirus</term><term>Homme</term><term>Humains</term><term>Lymphocyte T</term><term>Déterminant antigénique</term><term>Protéine A</term><term>Immunité cellulaire</term><term>Microbiologie</term><term>Séquence d'acides aminés</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-γ) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-γ-secreting T-cell response in HLA-A2<sup>+</sup> donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2<sup>+</sup> healthy donors or in HLA-A2- donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HLA-A2<sup>+</sup> SARS-CoV-infected patients.</div></front></TEI><affiliations><list><country><li>Danemark</li><li>Hong Kong</li><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></noRegion><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><name sortKey="He, Xiao Yan" sort="He, Xiao Yan" uniqKey="He X" first="Xiao-Yan" last="He">Xiao-Yan He</name><name sortKey="Pang, Xue Wen" sort="Pang, Xue Wen" uniqKey="Pang X" first="Xue-Wen" last="Pang">Xue-Wen Pang</name><name sortKey="Xu, Guo Bing" sort="Xu, Guo Bing" uniqKey="Xu G" first="Guo-Bing" last="Xu">Guo-Bing Xu</name><name sortKey="Yang, Rui Feng" sort="Yang, Rui Feng" uniqKey="Yang R" first="Rui-Feng" last="Yang">Rui-Feng Yang</name><name sortKey="Zhang, Hua Gang" sort="Zhang, Hua Gang" uniqKey="Zhang H" first="Hua-Gang" last="Zhang">Hua-Gang Zhang</name></country><country name="Hong Kong"><noRegion><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></noRegion><name sortKey="Cheng, Chak Sum Samuel" sort="Cheng, Chak Sum Samuel" uniqKey="Cheng C" first="Chak-Sum Samuel" last="Cheng">Chak-Sum Samuel Cheng</name><name sortKey="Jing Ju" sort="Jing Ju" uniqKey="Jing Ju" last="Jing Ju">JING JU</name><name sortKey="Li Meng" sort="Li Meng" uniqKey="Li Meng" last="Li Meng">LI MENG</name><name sortKey="Sin, Wan Yee Fion" sort="Sin, Wan Yee Fion" uniqKey="Sin W" first="Wan-Yee Fion" last="Sin">Wan-Yee Fion Sin</name><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name></country><country name="Danemark"><noRegion><name sortKey="Yang, Huang Hua" sort="Yang, Huang Hua" uniqKey="Yang H" first="Huang-Hua" last="Yang">Huang-Hua Yang</name></noRegion><name sortKey="Guo, Zhi Hong" sort="Guo, Zhi Hong" uniqKey="Guo Z" first="Zhi-Hong" last="Guo">Zhi-Hong Guo</name><name sortKey="Joice Na Lee Ng" sort="Joice Na Lee Ng" uniqKey="Joice Na Lee Ng" last="Joice Na Lee Ng">JOICE NA LEE NG</name><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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